By Nathan P. Kaplan, Nathan P. Colowick, John J. Langone, Helen Van Vunakis
The severely acclaimed laboratory regular, Methods in Enzymology, is among the such a lot hugely revered guides within the box of biochemistry. considering that 1955, every one quantity has been eagerly awaited, often consulted, and praised by means of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless appropriate this day - really a vital ebook for researchers in all fields of lifestyles sciences
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The severely acclaimed laboratory general, equipment in Enzymology, is likely one of the such a lot hugely revered guides within the box of biochemistry. on the grounds that 1955, every one quantity has been eagerly awaited, usually consulted, and praised by means of researchers and reviewers alike. The sequence includes a lot fabric nonetheless suitable at the present time - really a vital booklet for researchers in all fields of lifestyles sciences
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Extra resources for Immunochemical Techniques Part D: Selected Immunoassays
Tubes are incubated for 2 hr with slow shaking in a 37° water bath, followed by the addition of 150 fd of undiluted anti-IgG antiserum (determined to be in excess by pretitration). After 1 additional hour at 37° and 1 hr in an ice bath (or overnight at 4°), precipitates are collected by centrifugation at 8000 g (Sorvall HS4 rotor) for 25 rain. The supernatant is decanted and discarded, and the precipitates are counted. The antibody-specific binding is calculated for each serial dilution of antiserum according to Eq.
Holubar, and G. ), p. 215. Elsevier/North Holland, Amsterdam, 1978. 3 to 12 ng AFP/ml lated by precipitation with an equal volume of saturated cold neutral ammonium sulfate solution. 45-/~m Millipore membrane. Aliquots are kept at - 20° or lyophilized. Solid Phase Antibody. 5 mm) (Precision Plastics Co. 2 and TABLE IV PROTOCOL FOR THE SANDWICH ENZYME IMMUNOASSAY OF AFP 1. 1 ml of assay samples in duplicate into disposable polystyrene tubes 1-2 : Chicken serum (zero dose) 3-10: Standards 11- : Test and quality control samples.
Data were reproducedfrom Ref. 3 withpermission of the editor of Clinical Chemistry. 32 ONCOFETAL PROTEINS  R a d i o i m m u n o a s s a y By MARK J.  of Carcinoembryonic KRANTZ, SUZANNE LAFERTE, Antigen and N A O M I A R I E L Carcinoembryonic antigen (CEA) is an oncofetal glycoprotein expressed in the human fetal digestive system and in adenocarcinomas of the digestive system. It was first identified in colonic tumor tissue by means of antisera raised against human colonic tumor extracts and absorbed with normal tissue extracts in order to render the sera specific for tumor antigen(s)?